Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha antibody [HL3027] (GTX640465) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

ENaC Alpha antibody [HL3027] detects ENaC Alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ENaC Alpha stained by ENaC Alpha antibody [HL3027] (GTX640465) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ENaC Alpha antibody [HL3027] detects ENaC Alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
ENaC Alpha stained by ENaC Alpha antibody [HL3027] (GTX640465) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha (GTX640465) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.