Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EPHX1 antibody (GTX109360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1(GTX109360) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EPHX1 antibody detects EPHX1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
EPHX1 antibody (GTX109360) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1(GTX109360) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.