Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (23.81-0.37 nM). Coated protein was probed with EPO antibody (GTX100813) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

EPO antibody detects EPO protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
EPO antibody (GTX100813) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Recombinant human EPO protein (with glycosylation) expressed in CHO cells (100 ng) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody (GTX100813) diluted at 1:10000.

Recombinant mouse EPO protein (100 ng) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody (GTX100813) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody (GTX100813) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.