APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide of human ERK1/2.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 3 , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331](/templates/default/js/lightbox/images/loading.gif "Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331")
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GTX640331 IHC-P Image
ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat esophagus.ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX640331 IHC-P Image
ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis.Sample: Paraffin-embedded human oral carcinoma.ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX640331 IHC-P Image
ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX640331 ICC/IF Image
ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for ERK1/2 antibody [HL2946] (GTX640331). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ERK1/2 antibody [HL2946] (GTX640331). Be the first to share your experience with this product.
![Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331](/upload/website/prouct_img/small/GTX640331/GTX640331_T-45404_20240626_IHC-P_R_24070822_584.webp "Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331")
![Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331](/upload/website/prouct_img/small/GTX640331/GTX640331_T-45404_20240626_IHC-P_24070822_241.webp "Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331")
![Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331](/upload/website/prouct_img/small/GTX640331/GTX640331_T-45404_20240626_IHC-P_M_24070822_459.webp "Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331")
![Anti-ERK1/2 antibody [HL2946] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640331](/upload/website/prouct_img/small/GTX640331/GTX640331_T-45404_20240524_ICC_IF_24081300_511.webp "Anti-ERK1/2 antibody [HL2946] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640331")
![Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331](/upload/website/prouct_img/normal/GTX640331/GTX640331_T-45404_20240626_IHC-P_R_24070822_584.webp "Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331")
![Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331](/upload/website/prouct_img/normal/GTX640331/GTX640331_T-45404_20240626_IHC-P_24070822_241.webp "Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331")
![Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331](/upload/website/prouct_img/normal/GTX640331/GTX640331_T-45404_20240626_IHC-P_M_24070822_459.webp "Anti-ERK1/2 antibody [HL2946] used in IHC (Paraffin sections) (IHC-P). GTX640331")
![Anti-ERK1/2 antibody [HL2946] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640331](/upload/website/prouct_img/normal/GTX640331/GTX640331_T-45404_20240524_ICC_IF_24081300_511.webp "Anti-ERK1/2 antibody [HL2946] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640331")