Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat esophagus.
ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min