ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody

Cat. No. GTX24819
Cat. No.  GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
GTX24819 WB Image

WB analysis of NIH3T3 untreated (Lane 1) or treated with PDGF (Lane 2) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Loading: 5 ug

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Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
GTX24819 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

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Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
GTX24819 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

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Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
GTX24819 WB Image

WB (peptide competition) analysis of PC12 cells stimulated with 0.5 M sorbitol for 5 minutes (Lane 2-6) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

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Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
GTX24819 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50

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Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
GTX24819 WB Image

WB analysis of whole cell extracts (30 μg lysate) of MDA-MB-231 with treatment of EGF(100ng/ml for 15mins) (Lane 1), MDA-MB-231 (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HeLa (Lane 5) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Dilution : 1:1000

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Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P IHC IHC (Free Floating)
  • Reactivity
    Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Xenopus
See all ERK1 products