Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERO1L antibody (GTX112589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed Hep3B, using ERO1L(GTX112589) antibody at 1:500 dilution.

ERO1L antibody detects ERO1L protein by Western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract
B. 30 μg MCF-7 whole cell lysate/extract
10 % SDS-PAGE
ERO1L antibody (GTX112589) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ERO1L(GTX112589) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min