ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody (GTX108166) 7.5% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (GTX108166) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

ERR alpha antibody [N1], N-term detects ERR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ERR alpha antibody [N1], N-term detects ERR alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERR alpha antibody [N1], N-term detects ERR alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
12% SDS PAGE
GTX108166 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

ERR alpha antibody [N1], N-term detects ERR alpha protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

The data was published in the journal PLoS One in 2017. PMID: 29190800

The data was published in the journal PLoS One in 2015. PMID: 25781607

The data was published in the journal PLoS One in 2017. PMID: 29190800