Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ETS1 antibody [N3C1], Internal (GTX100639) diluted at 1:500 and competitor's antibody (sc-350) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ETS1 antibody [N3C1], Internal (GTX100639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.