Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETS2 antibody [HL2259] (GTX638311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

ETS2 antibody [HL2259] detects ETS2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ETS2 stained by ETS2 antibody [HL2259] (GTX638311) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Untreated (–) and treated (+) Raw 264.7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETS2 antibody [HL2259] (GTX638311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.