ETS2 antibody immunoprecipitates ETS2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 μg of ETS2 antibody (GTX104527) 7.5% SDS-PAGE The immunoprecipitated ETS2 protein was detected by ETS2 antibody (GTX104527) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

ETS2 antibody detects ETS2 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
ETS2 antibody (GTX104527) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ETS2(GTX104527) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ETS2 antibody (GTX104527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PTHrP promoter.

Untreated (–) and treated (+) Raw 264.7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETS2 antibody (GTX104527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Oncotarget in 2017. PMID: 28978027

The data was published in the journal PLoS Genet in 2019. PMID: 31306413