Name: EasyBlot anti Goat IgG (HRP)
Brand: GeneTex
SKU: GTX628547-01
Price: 199.00 USD

EasyBlot anti Goat IgG (HRP)

Cat. No. GTX628547-01

References 2

Cat. No. GTX628547-01

GTX628547-01 WB Image

EasyBlot anti-Goat IgG (HRP)
Comparison of western blot analysis with traditional secondary antibody (GTX228416-01 diluted at 1:5000) (1) and EasyBlot anti-Goat IgG kit (GTX628547-01 diluted at 1:2000) (2).
A. 0.2 μg native goat IgG
B. 0.1 μg native goat IgG
C. 1 μg denatured goat IgG
D. 2 μg denatured goat IgG
E. 4 μg denatured goat IgG
F. 8 μg denatured goat IgG

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GTX628547-01 WB Image

GST antibody immunoprecipitates GST-fused protein in IP experiments.
IP Sample: GST-fused protein expressed E. coli lysate
A. total lysate
B. Control with 1 μg of preimmune goat IgG
C. Immunoprecipitation of GST-fused protein by 1 μg of GST antibody (GTX26633)
12% SDS-PAGE
The immunoprecipitated GST-fuesd protein was detected by GST antibody (GTX26633) diluted at 1:10000.
EasyBlot anti-goat IgG (GTX628547-01) was used as a secondary reagent.

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Host

Mouse
Clonality

Monoclonal
Isotype

IgG2a
Applications

WB
Reactivity

Goat

Summary

EasyBlot anti-Goat IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of Goat IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to goat IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-goat IgG (Optimized for Prot A/G) (GTX228910-01) or EasyBloker (GTX425858) to minimize protein A/G contamination.