GeneTex
EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G)
Cat. No. GTX225857-01
Cat. No. GTX225857-01
GTX225857-01 WB Image
EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G) (GTX225857-01) was used as a secondary reagent for immunoprecipitation analysis.
HepG2 lysates were immunoprecipitated using 5 ug of normal rabbit IgG or ELP3 antibody [GT11811] (GTX631395).
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ApplicationsWB
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ReactivityMouse
Summary
EasyBlot anti-Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of mouse IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP).
The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.
The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.
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