Name: EasyBlot anti Rabbit IgG (HRP)
Brand: GeneTex
SKU: GTX221666-01
Price: 199.00 USD
Rating: 5 (1 reviews)

EasyBlot anti Rabbit IgG (HRP)

Cat. No. GTX221666-01

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Cat. No. GTX221666-01

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody, EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) and EasyBlot anti-Rabbit IgG kit (GTX225856-01). A,C,and E: E. coli lysate with overexpressed GST-tagged protein B,D,and F: IP result IP and 1st antibody: anti-GST antibody

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GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody (1) and EasyBlot anti-Rabbit IgG kit (GTX225856-01)(2). (A) Input, 20ug of HCT116 lysate. (B) and (C): HCT116 lysates were immunoprecipitated using 2.5 ug of normal rabbit IgG (B) or anti-p53 antibody (GTX102965)(C). The precipitated protein was detected by GTX102965 diluted at 1:10000.

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GTX221666-01 WB Image

GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736). The precipitated protein was detected by GTX110736 diluted at 1:3000.

A: EasyBlot
B: Goat anti-rabbit antibody

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GTX221666-01 WB Image

EasyBlot anti Rabbit IgG (HRP) antibody detects native-rabbit IgG by western blot analysis.Recombinant Protein (8 and 0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EasyBlot anti Rabbit IgG (HRP) antibody (GTX221666-01) at a dilution of 1:5000.

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Host

Mouse
Clonality

Monoclonal
Isotype

IgG1
Applications

WB
Reactivity

Rabbit

Summary

EasyBlot anti-Rabbit IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rabbit IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Rabbit IgG (Optimized for Prot A/G) (GTXGTX225856-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Rabbit IgG Agarose beads (GTX27019) instead of Protein A/G.