Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
zebrafish embryo
Predict Reactivity
Human (100%)| Mouse (99%)| Rat (99%)| Zebrafish (95%)| Rabbit (100%)| Bovine (100%)| Chicken (99%)| Japanese medaka (80%)| Xenopus tropicalis (98%)
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
The immunogen used to generate this antibody corresponds to zebrafish Ef1a
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11177741
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
eukaryotic translation elongation factor 1 alpha 1, like 1 , EFL1-alpha , chunp6927 , eef1a , ef1a , ik:tdsubc_2a3 , ik:tdsubc_2b3 , tdsubc_2a3 , wu:fa91c07 , wu:fa94b03 , wu:fi13b09 , xx:tdsubc_2a3 , xx:tdsubc_2b3
Cellular Localization
Cytoplasm
Background
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Database
Research Area