Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Enterovirus 71 VP1 protein, His tag (GTX138560-pro) (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Sandwich ELISA detection of recombinant Enterovirus 71 VP1 protein, His tag (GTX138560-pro) using antibodies as below.

Capture: Enterovirus 71 VP1 antibody [HL1929] (GTX637688) (5 μg/mL)
Detection: HRP-conjugated Enterovirus 71 VP1 antibody [HL1928] (GTX637687) (1 μg/mL)
Please notice that GTX637687 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637688. Please contact us for custom HRP-conjugated antibody.

Enterovirus 71 VP1 antibody [HL1929] detects Enterovirus 71 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) RD whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.