Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Enterovirus D68 VP1 antibody (GTX637898 [HL1997])
Detection: Enterovirus D68 VP1 antibody (GTX633770 [GT1843])

Samples (80 ng) :
1. Enterovirus D68 VP1 protein (GTX138561-pro)
2. Lysis buffer

Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Enterovirus D68 VP1 antibody (GTX633770 [GT1843])
Detection: Enterovirus D68 VP1 antibody (GTX637898 [HL1997])

Samples (80 ng) :
1. Enterovirus D68 VP1 protein (GTX138561-pro)
2. Lysis buffer

Enterovirus D68 VP1 antibody [GT1843] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT1843] (GTX633770) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT1843] (GTX633770) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Enterovirus D68 VP1 antibody [GT1843] detects Enterovirus D68 VP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Enterovirus D68 VP1 transfected 293T cell.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT1843] (GTX633770) diluted at 1:4000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below.

Capture: Enterovirus D68 VP1 antibody [GT1843] (GTX633770) (5 μg/mL)
Detection: Enterovirus D68 VP1 antibody [HL1997] (GTX637898) (1 μg/mL)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT1843] (GTX633770) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus D68 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus D68 VP1 antibody [GT1843] (GTX633770) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.