Application Note
IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. IF, FITC~conjugated antibodies 10-20 μg/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. FITC~conjugated antibodies, 0.2-1.0 μg/0.1 ml (1:200-1:1,000)
Calculated MW
Positive Control
Breast carcinoma
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.20 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Small cell lung carcinoma
Purification
Protein A purified
Conjugation
Fluorescein isothiocyanate (FITC)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Database
Research Area