Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Eph Receptor A3(GTX114067) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.