Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ephrin B2 antibody (GTX105582) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX105582 diluted at 1:1000