Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Estrogen Receptor alpha antibody [HL1081] detects Estrogen Receptor alpha protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Estrogen Receptor alpha antibody [HL1081] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Estrogen Receptor alpha antibody [HL1081] detects Estrogen Receptor alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min