Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

WB analysis of MCF-7 cell lysate by using GTX02805 Estrogen Receptor alpha antibody [SC1].
Lane 1-3 were triplicate.

ICC/IF analysis of PFA fixed MCF-7 cells by using GTX02805 Estrogen Receptor alpha antibody [SC1].
Dilution : 1-2 μg/ml

Estrogen Receptor alpha antibody [SC1] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:50000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.