Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (GTX127978) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Estrogen Receptor alpha antibody detects Estrogen Receptor alpha protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody (GTX127978) diluted at 1:500.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody (GTX127978) diluted at 1:2000 and competitor's antibody (MA1-310) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (GTX127978) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (GTX127978) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (GTX127978) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.