Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [4D2] (GTX70176) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using ER beta(GTX70176) antibody at 1:200 dilution.