Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody (GTX134663) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Estrogen Receptor beta antibody (GTX134663) diluted at 1:1000 and competitor's antibody (PPZ0506) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody (GTX134663) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.