Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] (GTX116036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] (GTX116036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using FABP4 (adipocyte)(GTX116036) antibody at 1:200 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FABP4(GTX116036) antibody at 1:200 dilution.

FABP4 antibody [N3C3] detects FABP4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
FABP4 stained by FABP4 antibody [N3C3] (GTX116036) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] (GTX116036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.