Wild-type (WT) and FADD knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FADD antibody (GTX100612) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FADD antibody (GTX100612) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with FADD antibody [N1C3] (GTX100612) diluted at 1:500 and competitor's antibody (sc-6035) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FADD antibody detects FADD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FADD stained by FADD antibody (GTX100612) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min