Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (GTX100764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (GTX100764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (GTX100764) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FAK antibody detects FAK protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FAK stained by FAK antibody (GTX100764) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

FAK antibody detects FAK protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
FAK stained by FAK antibody (GTX100764) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038