Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAM83B antibody [N1N2], N-term (GTX107222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FAM83B antibody [N1N2], N-term (GTX107222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using FAM83B(GTX107222) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min