Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:500.
Blue: Hoechst 33343 staining.

FANCJ antibody [N1N2], N-term detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
FANCJ antibody [N1N2], N-term (GTX100072) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.