Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL2(GTX104864) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

FBXL2 antibody [N1], N-term detects FBXL2 protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FBXL2 stained by FBXL2 antibody [N1], N-term (GTX104864) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

FBXL2 antibody [N1], N-term detects FBXL2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FBXL2 stained by FBXL2 antibody [N1], N-term (GTX104864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FBXL2 antibody [N1], N-term (GTX104864) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.