Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with FGFR4 antibody [N3C2], Internal (GTX112935) diluted at 1:500 and competitor's antibody (CST#2894) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR4 antibody [N3C2], Internal (GTX112935) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.