Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX100266) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX100266) diluted at 1:1000.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX100266) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.