Untreated (–) and treated (+) HUVEC whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FLRT2 antibody (GTX123309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using FLRT2(GTX123309) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min