Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody (GTX100308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody (GTX100308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
FOXA1 antibody (GTX100308) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXA1(GTX100308) antibody at 1:1000 dilution.

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.

FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 μg whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 μg of FOXA1 antibody (GTX100308) 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody (GTX100308) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FOXA1 antibody (GTX100308) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the 2022 in Epigenetics Chromatin. PMID: 35331302

The data was published in the 2022 in Epigenetics Chromatin. PMID: 35331302