Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FOXO3A antibody [C3], C-term detects FOXO3A protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FOXO3A antibody immunoprecipitates FOXO3A protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 μg of FOXO3A antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated FOXO3A protein was detected by FOXO3A antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using FOXO3A(GTX100277) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX100277 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.