Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FSTL1 antibody [N1C2] (GTX118133) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX118133 diluted at 1:1000