U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FSTL1 antibody [N1C3] (GTX118134) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FSTL1 antibody [N1C3] (GTX118134) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using FSTL1(GTX118134) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

FSTL1 antibody [N1C3] detects FSTL1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FSTL1 antibody [N1C3] (GTX118134) dilution: 1:250.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min