Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX104579) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FUBP1 protein stained by FUBP1 antibody (GTX104579) diluted at 1:500.
Blue: Hoechst 33342 staining.

FUBP-1 antibody immunoprecipitates FUBP-1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 μg of FUBP-1 antibody (GTX104579) 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody (GTX1104579) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX104579) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FUBP1 antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FUBP1 antibody (GTX104579) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

FUBP1 antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FUBP1 antibody (GTX104579) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX104579) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.