Rat tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody (GTX109830) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HepG2 conditioned medium (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody (GTX109830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Factor X(GTX109830) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Factor X antibody detects F10 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells
10 % SDS-PAGE
Factor X antibody (GTX109830) dilution: 1:5000

Immunofluorescence analysis of methanol-fixed HeLa, using Factor X(GTX109830) antibody at 1:200 dilution.