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Fast skeletal myosin light chain 1 antibody

Anti-Fast skeletal myosin light chain 1 antibody used in Western Blot (WB). GTX113092
Anti-Fast skeletal myosin light chain 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113092
Anti-Fast skeletal myosin light chain 1 antibody used in Western Blot (WB). GTX113092
Anti-Fast skeletal myosin light chain 1 antibody used in Western Blot (WB). GTX113092

Cat. No. GTX113092

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Myosin Light chain products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

mouse heart

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fast skeletal myosin light chain 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721790

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin light chain 1 , MLC1F , MLC3F

Background

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fast skeletal myosin light chain 1 antibody used in Western Blot (WB). GTX113092

GTX113092 WB Image

RD whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Fast skeletal myosin light chain 1 antibody (GTX113092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fast skeletal myosin light chain 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113092

GTX113092 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fast skeletal myosin light chain 1(GTX113092) antibody at 1:500 dilution.

Anti-Fast skeletal myosin light chain 1 antibody used in Western Blot (WB). GTX113092

GTX113092 WB Image

Sample (whole cell lysate)
A: 1ug mouse heart
B: 5ug mouse heart
15% SDS PAGE
GTX113092 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fast skeletal myosin light chain 1 antibody used in Western Blot (WB). GTX113092

GTX113092 WB Image

Fast skeletal myosin light chain 1 antibody detects Fast skeletal myosin light chain 1 protein by Western blot analysis. Rat tissue extracts (1 μg) was separated by 15 % SDS-PAGE, and the membrane was blotted with Fast skeletal myosin light chain 1 antibody (GTX113092) at a dilution of 1:20000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169