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Fc epsilon R1 alpha antibody [CRA1]

Overexpression
Anti-Fc epsilon R1 alpha antibody [CRA1] used in Flow cytometry (FACS). GTX00711
Anti-Fc epsilon R1 alpha antibody [CRA1] used in IHC (Frozen sections) (IHC-Fr). GTX00711

Cat. No. GTX00711

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CRA1

Isotype

 IgG2b

Application

 WB, IHC-P, IHC-Fr, FACS, IP, Activation

Reactivity

 Human, House musk shrew
Package
100 μg ($399)
See all Fc epsilon R1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB ~1 μg/ml
IHC-P ~1 μg/ml
IHC-Fr ~1 μg/ml
FACS 1-5 μg/ml|
IP Assay dependent
Activation Assay dependent

Note :

Activation
Promotion of migration of basophils
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant extracellular protein of human Fc epsilonR1alpha; (corresponding to amino acids Met-26-197, where signal peptide is 1-25).
Epitope: 26-110 amino acids

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgE receptor Ia , FCE1A , FcERI

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Fc epsilon R1 alpha antibody [CRA1] used in Flow cytometry (FACS). GTX00711

GTX00711 FACS Image

Overexpression

FACS analysis of CHO cells overexpressing human Fc epsilon R1 alpha protein using GTX00711 Fc epsilon R1 alpha antibody [CRA1].

Anti-Fc epsilon R1 alpha antibody [CRA1] used in IHC (Frozen sections) (IHC-Fr). GTX00711

GTX00711 IHC-Fr Image

IHC-Fr analysis of Human atopic dermatis lesional skin tissue section using GTX00711 Fc epsilon R1 alpha antibody [CRA1].
Fixation : Aceton

REFERENCE

Clone Reference

Ito R et al. J Immunol. 2013; Establishment of a human allergy model using human IL-3/GM-CSF-transgenic NOG mice.
Application : IHC-P
Reactivity : Human
Ono HK et al. FEMS Immunol Med Microbiol. 2012; Submucosal mast cells in the gastrointestinal tract are a target of staphylococcal enterotoxin type A.
Application : IHC-Fr
Reactivity : House musk shrew
Takai T et al. Biosci Biotechnol Biochem. 2001; Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli.
Application : WB
Reactivity : Human
Hasegawa S et al. Blood. 1999; Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes.
Application : FACS
Reactivity : Human
Suzuki K et al. Mol Immunol. 1998; 35 (5):259-70 The Fc receptor (FcR) gamma subunit is essential for IgE-binding activity of cell-surface expressed chimeric receptor molecules constructed from human high-affinity IgE receptor (Fc epsilon RI) alpha and FcR gamma subunits.
Application : WB, IP
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399