Sandwich ELISA analysis of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml, 100ul) using GTX15737 Fibronectin antibody [3F12] (detection antibody) at 1 μg/mL and fibronectin polyclonal antibody (capture antibody).

WB analysis of 30 μg of the Caco-2 whole cell lysates using GTX15737 Fibronectin antibody [3F12].
Dilution : 2 μg/ml

ICC/IF analysis of HepG2 cells using GTX15737 Fibronectin antibody [3F12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 overnight at 4 ºC

WB analysis of 25 ug of human plasma lysates using GTX15737 Fibronectin antibody [3F12].
Dilution : 1:300