Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term (GTX112794) 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Competitive ELISA detection of human fibronectin in plasma using GTX112794. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 μg/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2,000 and used to detect the primary antibody.

Indirect ELISA analysis was performed by coating plate with 100 μL of recombinant Fibronectin protein at concentration of 10 μg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GTX112794) at rangeing from 0.5 to 140 ng/mL.

Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin (GTX112794) antibody at 1:200 dilution.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

The data was published in the journal Oncotarget in 2016.PMID: 26840566

The data was published in the journal Oncotarget in 2016.PMID: 26840566

The data was published in the journal Nat Commun in 2017.PMID: 28102225

The data was published in the journal Nat Commun in 2017.PMID: 28102225

The data was published in the journal Oncotarget in 2015. PMID: 25460509

The data was published in the journal Nat Commun in 2015. PMID: 26245647

The data was published in the journal Nat Commun in 2015. PMID: 26245647