Firefly Luciferase antibody detects Firefly Luciferase protein by immunohistochemical analysis.
Sample: Firefly Luciferase-transfected 293T cell FFPE Cell Pellet Block.
Green: Firefly Luciferase stained by Firefly Luciferase antibody (GTX125849) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody (GTX125849) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Firefly Luciferase stained by Firefly Luciferase antibody (GTX125849) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal J Control Release in 2014. PMID: 25449884