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Flotillin 2 antibody [C3], C-term

Orthogonal
Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Immunoprecipitation (IP). GTX100279

Cat. No. GTX100279

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Flotillin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLa , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240819

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flotillin 2 , ECS-1 , ECS1 , ESA , ESA1 , M17S1

Cellular Localization

Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

GTX100279 WB Image

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10% SDS-PAGE
Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

GTX100279 WB Image

Orthogonal

293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Flotillin 2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100279

GTX100279 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.

Anti-Flotillin 2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100279

GTX100279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

GTX100279 WB Image

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10% SDS-PAGE
Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

GTX100279 WB Image

Flotillin 2 antibody detects Flotillin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody (GTX100279) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Observed Flotillin 2 protein is larger than the predicted M.W., possibly due to post-translational modifications.
Reference: PLoS One. 2009 Aug 4;4(8):e6497.

Anti-Flotillin 2 antibody [C3], C-term used in Immunoprecipitation (IP). GTX100279

GTX100279 IP Image

Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 μg of Flotillin 2 antibody [C3], C-term (GTX100279).
Western blot analysis was performed using Flotillin 2 antibody [C3], C-term (GTX100279).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169