Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Flotillin 2(GTX114411) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Flotillin 2 antibody [N1C1] immunoprecipitates Flotillin 2 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Flotillin 2 protein by 4 μg Flotillin 2 antibody [N1C1] (GTX114411)
10 % SDS-PAGE
The immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody [N1C1] (GTX114411) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
Flotillin-2 antibody
GTX114411 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114411 diluted at 1:1000