Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Boiled and unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.