APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.03μg/ml |
| 3.75μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89022-PEP).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1
Background
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89022 ELISA Image
ELISA analysis of Fumarate hydratase protein using GTX89022 Fumarate hydratase antibody, Internal.
Capture : GTX110128 (2μg/ml)
Detection : GTX89022 (1.5μg/ml)
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GTX89022 WB Image
WB analysis of human kidney lysate using GTX89022 Fumarate hydratase antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89022 WB Image
WB analysis of HEK293 overexpressing Fumarate hydratase (mock transfection in first lane) using GTX89022 Fumarate hydratase antibody, Internal.
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GTX89022 IHC-P Image
IHC-P analysis of human skeletal muscle using GTX89022 Fumarate hydratase antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89022 IHC-P Image
IHC-P analysis of human heart using GTX89022 Fumarate hydratase antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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REFERENCE
There are currently no references for Fumarate hydratase antibody, Internal (GTX89022). Be the first to share your publications with this product.
REVIEW
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