Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of G9a protein from HeLa whole cell extracts using 5 μg of G9a antibody (GTX128164).
Western blot analysis was performed using G9a antibody (GTX128164) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

G9a antibody detects G9a protein at nucleus in human rectal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human rectal carcinoma.
G9a antibody (GTX128164) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

G9a antibody detects EHMT2 protein at on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
G9a antibody (GTX128164) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

G9a antibody detects EHMT2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min.
Green: EHMT2 protein stained by G9a antibody (GTX128164) diluted at 1:500.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000.

G9a antibody detects G9a protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
G9a stained by G9a antibody (GTX128164) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min