Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

G9a antibody detects G9a protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:3000.

G9a antibody detects G9a protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
G9a antibody (GTX129153) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

G9a antibody detects G9a protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: G9a protein stained by G9a antibody (GTX129153) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

G9a antibody immunoprecipitates G9a protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of G9a by 3 μg of G9a antibody (GTX129153) 5% SDS-PAGE The immunoprecipitated G9a protein was detected by G9a antibody (GTX129153) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-G9a antibody. The precipitated DNA was detected by PCR with primer set targeting to FXN.